The UGA-42 Caliburn is a galvanometer based localized illumination system for laser induced damage in cells, tissue, and organisms. The damage is done under highly controlled parameters in points or selected regions in the field of view of the microscope.
The UGA-42 Caliburn uses pulsed UV lasers and features built-in motorized laser focus, safety shutter, interlock, and digital laser modulation. A built-in attenuator ensures fine power adjustment.
The photomanipulation and control software runs independently, allowing uninterrupted imaging during photomanipulation.
For moving or changing samples, the ablation can be done in real-time (“Click & Fire” mode), with predetermined temporal parameters, at locations chosen by the user during the experiment. For fixed samples, in addition to the “Click & Fire” option, sequences of ROIs with user-designed spatial and temporal parameters can be set up prior to the experiment (“Sequence” mode) and run at the click of a button or synchronized with other events via TTL pulses.
The Caliburn is a versatile add-on to most imaging (confocal, spinning disk, wide-field, etc.) microscopes.