Flexible, modular, large spot localized illumination system

  • Scanner-based illumination system
  • Selectable spot shapes and sizes
  • Dynamic, localized illumination in the field of view of the microscope
  • User-designed spatial & temporal illumination control
  • Available for a large number of research microscope models
  • Easily integrated into imaging and electrophysiology rigs
  • Wide variety of applications

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The UGA-42 Geo is a localized illumination system based on an XY galvanometer scanner and designed for dynamic illumination of large spots or selected regions in field of view of the microscope. The system can be integrated into a variety of imaging (confocal, spinning disk, wide field, etc.) and electrophysiology rigs.

The Geo features multiple factory preset spot shapes and sizes offering the possibility to illuminate larger areas (eg. a whole cell or part of a cell) in a single shot, partially eliminating the need for scanning. Compatible with fiber-coupled lasers or laser launches (eg. LDI). All lasers include built-in safety shutters and interlocks and can be analog and digital modulated.

The software runs in parallel with and independent of the imaging software, allowing uninterrupted imaging during photostimulation.

For moving or changing samples, the illumination can be done in real-time, with predetermined temporal parameters, at locations chosen at will by the user during the experiment. For fixed samples, the illumination is set up prior to the experiment as sequences of regions of interest (ROIs) with user-designed spatial and temporal parameters and run at the click of a button or synchronized with other events in the experiment via TTL pulses.

Laser Coupling Optical fiber
Laser Type Diode lasers and laser launches; compatible with the LDI
Spot Size Various factory set spot shapes (circles, squares, rectangles, donuts) of different sizes to choose from
Switching Time < 1 ms between any locations in the field of view
Number of Laser Couplings on the Geo One
Maximum Number of Lasers per System Up to 4 when combined with other UGA-42 modules
Maximum Number of Lasers per Experiment Up to 4
Switching Time Between Lasers Microsecond range
ROI Geometries Circles, straight lines, rectangles, and user-defined polygons & lines
Galvanometer Calibration Autocalibration algorithm
Synchronization with Other Devices Bidirectional TTL communication (slave or master) with other devices
Laser Modulation Analog and digital
Built-In Safety Shutter Yes
Built-In Interlock Yes
  • Add-on optical module available for most of the current models of research microscopes
  • Simultaneous and independent spot illumination and imaging
  • Quiet operation for integration into electrophysiology rigs
  • Variety of spot shapes (circles, squares, rectangles, and donuts) of different sizes available to choose from to satisfy different experimental conditions and partly eliminate scanning
  • The laser spot can be located anywhere in the field of view of the microscope or further scanned over user-designed regions of interest
  • ROI geometries include standard shapes (straight lines, circles, rectangles) or user-designed polygons and lines
  • Autocalibration algorithm
  • Fast switching between any two positions in the field (< 1 ms)
  • “Click-and-Fire” mode for real time illumination in points or user-designed ROIs
  • “Sequence mode” programmable illumination with user-designed spatial and temporal control
  • Versatile and user-friendly editing functions for spatial and temporal sequence elements
  • Runs in parallel with and independent of any imaging software
  • Communication protocols available for Metamorph, Zeiss ZEN2, ZENBlue, ZENBlack, Nikon Elements, Micromanager
  • Compatible with fiber-coupled lasers and laser launches; compatible with the LDI
  • Analog and digital laser modulation
  • Lasers feature built-in safety shutters and interlocks
  • Two TTL inputs and two TTL outputs for synchronization as slave or master with other devices
  • Manual or TTL sequence start
  • Synchronization of individual ROIs with other events in the experiment
  • Can be combined with the UGA-42 Firefly and the UGA-42 Caliburn to increase the functionality of the rig
  • Uncaging/Photolysis
  • Optogenetics
  • Neural mapping
  • Photoconversion/Photoswitching
  • FRAP/Photobleaching
  • FRET
  • Selective, localized illumination of photosensitive materials
  • Localized heating and temperature-jump

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