TwinCam LS Image Splitter

Affordable and user-friendly image splitter that enables two-camera, simultaneous dual-emission imaging from a single microscope port

  • Easy to align and maintain
  • Ideal for FRET and wide-field large sensor simultaneous dual-channel imaging

In Stock

The TwinCam LS Image Splitter has been designed as a convenient solution for simultaneous two-channel imaging without sacrificing the field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially.  However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the TwinCam LS allows two images to be acquired simultaneously.

This image splitter enables pixel alignment between two cameras coupled to a single microscope port. The base unit accommodates a standard Cairn filter cube allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Standard Spectral Range450 nm – 900 nm
Dimensions348 mm (L) x 191.5 mm (W) x 72 mm (H)
Weight1.8 kg
Emission Filter Size25 mm
Dichroic Mirror Size25.5 mm x 36.0 mm
26.0 mm x 38.0 mmup to 3.0 mm thick, UltraFlat dichroics highly recommended
Camera Size SupportedUp to 22 mm diagonal
Supports Large Sensors up to 22 mm DiagonalEnables the largest FOV possible
User Exchangeable Dichroic MirrorsEasily reconfigure the system for different experiments
Adjustable Input AperturePrecisely define the desired field of view; also supports cropped sensor mode found in many EMCCD cameras
Supports Dichroic Mirrors up to 3 mm thickDistortion-free images
Stable AlignmentLong-term time-lapse imaging
Optional Auxiliary Filter HoldersAccommodates ND filters, chromatic correction lenses, supplementary emission filters or lens for multiplane microscopy
Independent Camera FocusCorrect Chromatic aberrations from microscope, or create multiplane imaging


  • Förster Resonance Energy Transfer (FRET)
  • Simultaneous use of multiple dyes or genetic markers
  • Ratiometric imaging
  • Polarization studies
  • Simultaneous transmitted light and fluorescence imaging
  • Simultaneous high-speed and high-resolution imaging
  • Multifocal Plane Microscopy

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