TwinCam LS Image Splitter

Affordable and user-friendly image splitter that enables two-camera, simultaneous dual-emission imaging from a single microscope port

  • Easy to align and maintain
  • Ideal for FRET and wide-field large sensor simultaneous dual-channel imaging

The TwinCam LS Image Splitter has been designed as a convenient solution for simultaneous two-channel imaging without sacrificing the field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially.  However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the TwinCam LS allows two images to be acquired simultaneously.

This image splitter enables pixel alignment between two cameras coupled to a single microscope port. The base unit accommodates a standard Cairn filter cube allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Standard Spectral Range 450 nm – 900 nm
Dimensions 348 mm (L) x 191.5 mm (W) x 72 mm (H)
Weight 1.8 kg
Emission Filter Size 25 mm
Dichroic Mirror Size 25.5 mm x 36.0 mm
26.0 mm x 38.0 mmup to 3.0 mm thick, UltraFlat dichroics highly recommended
Camera Size Supported Up to 22 mm diagonal
Supports Large Sensors up to 22 mm Diagonal Enables the largest FOV possible
User Exchangeable Dichroic Mirrors Easily reconfigure the system for different experiments
Adjustable Input Aperture Precisely define the desired field of view; also supports cropped sensor mode found in many EMCCD cameras
Supports Dichroic Mirrors up to 3 mm thick Distortion-free images
Stable Alignment Long-term time-lapse imaging
Optional Auxiliary Filter Holders Accommodates ND filters, chromatic correction lenses, supplementary emission filters or lens for multiplane microscopy
Independent Camera Focus Correct Chromatic aberrations from microscope, or create multiplane imaging


  • Förster Resonance Energy Transfer (FRET)
  • Simultaneous use of multiple dyes or genetic markers
  • Ratiometric imaging
  • Polarization studies
  • Simultaneous transmitted light and fluorescence imaging
  • Simultaneous high-speed and high-resolution imaging
  • Multifocal Plane Microscopy

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