OptoSplit II LS Image Splitter

Easy-to-align image splitter that enables a single camera to record images simultaneously at two different emission wavelengths

  • User-friendly design requires minimal maintenance and makes alignment simple
  • Excellent for use in FRET, TIRF, and spinning disk confocal applications
  • Compatible with all major brands of camera, including large sCMOS chips

In Stock

The OptoSplit II LS has been designed as a convenient, inexpensive solution for simultaneous dual wavelength imaging. Traditionally, dual-wavelength imaging experiments required that two images be captured sequentially. However, because the two images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures. To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit II allows both images to be acquired simultaneously.

The images captured by the OptoSplit II contain information from two different emission bands, and can be processed using commercially available software packages. Alternatively, the images can be processed manually. While it is optimized for coupling to a scientific microscope, the OptoSplit II can also be used with camera lenses or any other system of lenses that produce an image plane of suitable size.

Standard Spectral Range450 nm – 900 nm
Dimensions110 mm (L) x 261 mm (W) x 79 mm (H)
Weight1.26 kg
Emission Filter Size25 mm
Dichroic Mirror Size25.5 mm x 36.0 mm
26.0 mm x 38.0 mm2.0 mm thick UltraFlat dichroics highly recommended
Camera Size SupportedUp to 22 mm diagonal


Supports large sensors up to 22 mm diagonalLarge field of view, including sCMOS detectors
User exchangeable dichroic mirrorsEasily reconfigure system for different experiments
Adjustable input aperturePrecise definition of the desired field of view; also supports cropped sensor mode found in many EMCCD cameras
Supports dichroic mirrors up to 3 mm thickDistortion-free images
Stable alignmentLong-term time-lapse imaging
Optional auxiliary filter holdersAccommodates ND filters, chromatic correction lenses, supplementary emission filters, or lens for multiplane microscopy
  • Ratiometric, calcium, voltage, and pH imaging
  • Dual probe widefield imaging
  • Förster Resonance Energy Transfer (FRET)
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal microscopy
  • Polarization/Anisotropy
  • Combined fluorescence/transmitted light microscopy
  • Multifocal plane microscopy

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