MultiCam LS Image Splitter

Highly flexible image splitter that enables multi-camera, simultaneous multi-channel imaging from a single microscope port

  • Supports up to 4 channels of varying polarity, emission wavelength, and depth of focus
  • Essential equipment for researchers wanting to push  the limits of simultaneous multi-channel imaging

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The MultiCam LS Image Splitter has been designed as a convenient solution for simultaneous multi-channel imaging without sacrificing field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially. However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the MultiCam LS allows multiple images to be acquired simultaneously.

This image splitter enables pixel alignment between multiple cameras coupled to a single microscope port. The base unit accommodates multiple Cairn filter cubes allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Standard Spectral Range 450 nm – 900 nm
Dimensions Variable depending on final configuration
Weight Variable depending on final configuration
Emission Filter Size 25 mm
Dichroic Mirror Size 25.5 mm x 36.0 mm
26.0 mm x 38.0 mmup to 3.0 mm thick, UltraFlat dichroics highly recommended
Camera Size Supported Up to 22 mm diagonal
Standard configuration supports up to 4 cameras Enables up to 4 full FOV images to be acquired simultaneously
Custom configuration supports up to 8 cameras Additional flexibility for combining modalities such as polarization and fluorescence in a single system
Supports large sensors up to 22 mm diagonal Large field of view, including sCMOS detectors
User exchangeable dichroic mirrors Easily reconfigure system for different experiments
Adjustable input aperture Precisely define the desired field of view; also supports cropped sensor mode found in many EMCCD cameras
Supports dichroic mirrors up to 3 mm thick Ensures a flat reflection surface and distortion-free images
Stable alignment Long-term time-lapse imaging
Optional auxiliary filter holders Accommodates ND filters, chromatic correction lenses, supplementary emission filters, or lenses for multiplane microscopy
Independent camera focus Correct Chromatic aberrations from microscope, or create multiplane imaging
  • Förster Resonance Energy Transfer (FRET)
  • Simultaneous use of multiple dyes or genetic markers
  • Ratiometric imaging
  • Polarization studies
  • Simultaneous transmitted light and fluorescence imaging
  • Simultaneous high speed and high resolution imaging
  • Multifocal plane microscopy

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