OptoSplit III LS Image Splitter

Easy-to-align image splitter that enables a single camera to record images simultaneously at three different emission wavelengths

  • User-friendly design requires minimal maintenance and makes alignment simple
  • Excellent for use in FRET, TIRF, and spinning disk confocal applications
  • Compatible with all major brands of camera, including large sCMOS chips

The OptoSplit III LS has been designed as a convenient, inexpensive solution for simultaneous multi-wavelength imaging. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially.  However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit III allows three images to be acquired simultaneously.

The images captured by the OptoSplit III contain information from three different emission bands, and can be processed using commercially available software packages. Alternatively, the images can be processes manually.  While it is optimized for coupling to a scientific microscope, the OptoSplit III can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size.

Standard Spectral Range

450 nm – 900 nm


122 mm (L) x 374 mm (W) x 79 mm (H)


1.80 kg

Emission filter size

25 mm

Dichroic mirror size

25.5 mm x 36.0 mm
26.0 mm x 38.0 mm

2.0 mm thick, UltraFlat dichroics highly recommended

Supports large sensors up to 22 mm diagonal

Enables the largest possible field of view

User exchangeable dichroic mirrors

Easily reconfigure system for different experiments

Adjustable input aperture

Precisely define the required field of view; also supports cropped sensor mode found in many EMCCD cameras

Supports dichroic mirrors up to 3 mm thick

Ensures a flat reflection surface and distortion-free images

Stable alignment

Enables long-term time-lapse imaging without the need to realign during the experiment

Optional auxiliary filter holders

Ability to balance emission intensity between the multiple channels or add emission; accommodates ND filters and chromatic correction lenses

  • Ratiometric, calcium, voltage & pH imaging
  • Triple probe widefield imaging
  • Förster Resonance Energy Transfer (FRET)
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal microscopy
  • Polarisation/Anisotropy
  • Combined fluorescence/transmitted light microscopy


Additional Information

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