The UGA-42 Geo is a localized illumination system based on an XY galvanometer scanner and designed for dynamic illumination of large spots or selected regions in field of view of the microscope. The system can be integrated into a variety of imaging (confocal, spinning disk, wide field, etc.) and electrophysiology rigs.
The Geo features multiple factory preset spot shapes and sizes offering the possibility to illuminate larger areas (eg. a whole cell or part of a cell) in a single shot, partially eliminating the need for scanning. Compatible with fiber-coupled lasers or laser launches (eg. LDI). All lasers include built-in safety shutters and interlocks and can be analog and digital modulated.
The software runs in parallel with and independent of the imaging software, allowing uninterrupted imaging during photostimulation.
For moving or changing samples, the illumination can be done in real-time, with predetermined temporal parameters, at locations chosen at will by the user during the experiment. For fixed samples, the illumination is set up prior to the experiment as sequences of regions of interest (ROIs) with user-designed spatial and temporal parameters and run at the click of a button or synchronized with other events in the experiment via TTL pulses.