OptoLED

Ultra-stable, high-intensity, modular illuminator

  • Independently drives two LEDs over a wide range of currents
  • Well-suited for epi-fluorescence, transmitted light microscopy, macroscopy, and optogenetics

With traditional light sources, the long-term drift in light intensity may negatively impact quantitative imaging experiments.  The OptoLED solves this problem with ultra-high stability, digital vibration-free switching, and rapid intensity modulation. The ability to “gate” the light source directly from the camera and thus only expose the specimen while the camera is integrating reduces photobleaching and also prevents bleedthrough during frame readout.

The OptoLED is capable of driving two LEDs independently, over a wide range of currents. For pulsed illumination, switching times of less than 100 nanoseconds are achievable. Digital inputs are provided to switch each LED on and off independently, and sockets are provided to allow additional synchronization with the camera to reduce phototoxicity or motion artifacts.  Optical feedback is included to prevent changes in light output that would otherwise result from variation in LED temperature.

Dimensions

Power supply – 280 mm x 304 mm x 65 mm

Remote slider – 87 mm x 144 mm x 35 mm

LED head – 46 mm x 46 mm x 73 mm

LEDs available

355nm, 365nm, 385nm, 405nm, 440nm, 455nm, 470nm, 490nm, 505nm, 525nm, 550nm, 565nm, 590nm, 617nm, 627nm and white with multi-element LED heads for transmitted light; for the 355 nm LED, post-filter feedback allows stable high Ca2+ Fura-2 imaging

Maximum output current

1A, 2A or 5A, selectable by internal jumper links

Optical switching times

<100 nsec

Feedback response time

<50 μsec

Analog voltage range

0 to +10V

FeatureBenefit
Optical feedback

Ensures excellent intensity and wavelength stability

Digital control

Instantaneous vibration-free switching

Long life

Minimizes downtime and long term costs

Variable intensity

Precise experimental control

Fully modular system

Accommodates the expanding range of LED wavelengths

Interfaces with single, dual, triple and quad couplings available for Nikon, Olympus, Leica and Zeiss microscopes

Integrates easily into a variety of experimental arrangements

Convenient front panel control

Easily accessible complete control of on/off and intensity

Optional remote slider

Complete system control in small, convenient hand controller

  • Multi-wavelength fluorescence microscopy
  • Visible/IR transmitted light microscopy
  • Optogenetics using fibres or epi-illumination
  • Macro fluorescence imaging/optical mapping
  • Flash photolysis
  • High speed Fura2 calcium imaging

Additional Information


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