MultiCam LS Image Splitter

Highly flexible image splitter that enables multi-camera, simultaneous multi-channel imaging from a single microscope port
  • Supports up to 8 channels of varying polarity, emission wavelength, and depth of focus
  • Excellent for use by researchers who are pushing the limits of simultaneous multi-channel imaging

The MultiCam LS Image Splitter has been designed as a convenient solution for simultaneous multi-channel imaging without sacrificing field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially. However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the MultiCam LS allows multiple images to be acquired simultaneously.

This image splitter enables pixel alignment between multiple cameras coupled to a single microscope port. The base unit accommodates multiple Cairn filter cubes allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Standard Spectral Range

450 nm – 900 nm


Variable depending on final configuration


Variable depending on final configuration

Emission filter size

25 mm

Dichroic mirror size

25.5 mm x 36.0 mm
26.0 mm x 38.0 mm

2.0 mm thick, UltraFlat dichroics highly recommended

Camera size supported

Up to 22 mm diagonal

Standard configuration supports up to 4 cameras

Enables up to 4 full FOV images to be acquired simultaneously

Custom configuration supports up to 8 cameras

Additional flexibility for combining modalities such as polarization and fluorescence in a single system

Supports large sensors up to 22 mm diagonal

Enables the largest FOV possible

User exchangeable dichroic mirrors

Easily reconfigure system for different experiments

Adjustable input aperture

Precisely define the required field of view; also supports cropped sensor mode found in many EMCCD cameras

Supports dichroic mirrors up to 3 mm thick

Ensures a flat reflection surface and distortion-free images

Stable alignment

Enables long-term time-lapse imaging without the need to realign during the experiment

Optional auxiliary filter holders

Ability to balance emission intensity between the multiple channels or add emission; accommodates ND filters and chromatic correction lenses

Independent camera focus

Correct chromatic aberrations from the microscope

  • Förster Resonance Energy Transfer (FRET)
  • Simultaneous use of multiple dyes or genetic markers
  • Ratiometric imaging
  • Polarization studies
  • Simultaneous transmitted light and fluorescence imaging
  • Simultaneous high speed and high resolution imaging
  • Simultaneous multi Z depth imaging

Additional Information

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