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DeepSIM Product Overview

Launched in October 2021, DeepSIM is a super resolution module developed to enhance the imaging capabilities of high end microscope systems.

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Specifications

Find specifications for DeepSIM below. Note that we can also work with you to provide solutions for your unique needs.

DeepSIM stand-alone  DeepSIM X-Light
FOV 1024x1024pixel (66×66 µm 100X | 333×333µm 20X) 1024x1024pixel (66×66 µm 100X | 333×333µm 20X)
Resolution Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45) ution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
DeepSIM Acquisition speed 13fps (1024x1024px) 13fps (1024x1024px)
Laser spectral range Excitation: 400-750 nm; emission: 400-850 nm Excitation: 400-750 nm; emission: 400-850 nm
Objective specifications from 20X to 100X magnification range -high numerical aperture (NA) -plan apochromat correction from 20X to 100X magnification range -high numerical aperture (NA) -plan apochromat correction
Camera compatibility Any triggerable camera having 6.5 µm pixel size Any triggerable camera having 6.5 µm pixel size
Spinning disk upgrade Stand-alone solution Add-on compatible with CrestOptics X-Light V3
Imaging modalities Super-resolution DeepSIM | Widefield Super-resolution DeepSIM | Confocal spinning disk X-light V3 | Widefield
Upgradable microscope configuration Upright and inverted configurations Inverted configuration
Software µManager /VisiView® / NIS Elements µManager /VisiView® / NIS Elements
Installation Conditions Temperature 23 ± 5°C, Humidity 70% RH or less Temperature 23 ± 5°C, Humidity 70% RH or less
Weight 50.7 lbs | 23Kg 44 lbs | 20Kg
Dimensions 13.8 (w) x 20.2 (L) x 11.4 (h) inches 352.0 (w) 514.0 (L) x 290.5 (h) mm 14.0 (w) x 17.1 (L) x 11.4 (h) inches 356.0 (w) 435.0 (L) x 290.5 (h) mm

Features

Find the unique features of the DeepSIM below.

Compact and stable Can be reconfigured rapidly and easily for different applications
Supplied with infinity-corrected tube lens and objectives from multiple sources, including Olympus and Nikon Simultaneous imaging with multiple cameras and large format sensors (>25mm diagonal)
Extensible, only buy (or build) what you need, as you need it Sustainable, can be supported and maintained by any competent microscopist or technician
Direct interface with parts from other manufacturers (e.g. Thorlabs) for rapid development of new techniques Brand agnostic, works with existing illuminators, cameras, stages, objectives, filters, and lenses from any manufacturer
Unrivalled access to optical train, laser pointer test kit available for alignment and validation Easy to clean or autoclave
Transportable – suitable for tradeshows, teaching courses and secondments No eye pieces – COVID-19 / multi-user friendly

 

Scientific Applications & Techniques

DeepSIM is used in some of the worlds most advanced laboratories in a wide range of applications and techniques. These include those listed below.

  • Wide-field fluorescence
  • Phase contrast
  • Fluorescence Lifetime Imaging (FLIM)
  • Simultaneous/ratiometric calcium and voltage imaging
  • High content imaging
  • Single molecule and super resolution STORM/PALM/SIM
  • Total Internal Reflection Fluorescence (TIRF)
  • Fluorescence Recovery After Photobleaching (FRAP) and optogenetics
  • Electrophysiology
  • Bioluminescence
  • Single Plane Illumination Microscopy (SPIM)
  • Containment labs (compact and easy to sterilise)
  • Polarisation studies (anisotropy)

Peer Reviewed in Scientific Publications

Our products are used in some of the most pioneering labs across the world. Here are some of the scientific citations we’ve received.

Our Products are Used in the Nation's Most Advanced Labs

Our LDI products are used in some of the nation’s most advanced laboratories. Here are a few.

Request A Quote

Contact us for a free quote. Need something bespoke? We can work with you to configure a specialized range of laser lines and options.

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