The X-Light V2 is a high-performance confocal imaging system ideally suited for both core imaging facilities and individual research labs. This spinning disk confocal system can seamlessly switch between widefield, confocal, and Super-resolution imaging (with the optional VCS add-on) on the same microscope.
The X-Light V2 comes standard with a 5-position motorized dichroic wheel, 8-position motorized emission filter wheel, motorized bypass mode and exchangeable disk boxes. An 8 position motorized excitation wheel is an available option. The X-Light V2 has has a large field of view it works with both EMCCDs for the sensitive, low-light applications as well as larger-format sCMOS cameras for higher resolution imaging. The X-Light V2 is compatible with the new high power, low cost LDI laser illuminator.
”Chloroplasts moving inside fresh-water plant Elodea canadeus. Z-Projection of 19 slices at 1um intervals; 10ms exposure of each slice and 500ms between 3D captures. Images taken at AQLM, MBL 2016”
|Confocal acquisition time|
< 200 microseconds
Widefield – spinning disk out of optical pathway via motorized bypass mode.
|Camera size supported|
Double pattern disk – up to 12 mm x 12 mm FOV (17mm diagonal)
|Disk pinhole size options|
Double pattern disk – 40 micron and 70 micron pinholes
Other patterns available upon request
25.5 mm x 36 mm x 2 mm (Ultraflat recommended)
|Emission filter wheel|
Automated 8-position wheel
|Emission filter size|
25 mm diameter
|Supported input fiber|
1.5 mm, 0.39NA multimode fiber with SMA adapter
|Supports both EMCCDs and sCMOS camera|
Functions well in both high-sensitivity and high-speed applications
|Works with single and multimode lasers sources|
Unsurpassed flexibility and a superior price to performance ratio
|15,000 RPM disc speed|
No need to synchronize with camera to avoid non-uniform acquisition intensities due to full field of view capture in less than 200us
|Motorized bypass mode|
Allows both confocal and widefield imaging from the same microscope port with the same camera.
|Easy to use disk focus mechanism|
Ensures the disk is at the proper image plane
- Live cell confocal imaging
- 3D optical sectioning
- Simultaneous multi-channel imaging (with optional emission splitting systems)
- Super-resolution imaging with optional VCS add-on