Short Term Stability of Light Sources

PhotoFluor II run at 60% output to match 100% power levels of the 120 W metal halide source. Light guide to collimating adapter to 0.5 ND filter to HQ470/40x filter to silicon detector, measurements by Newport 1830C followed by A/D conversion. Sampling at 2000 Hz for five seconds. 49004 filter set used with Tetraspeck slide. 20 X Zeiss objective.

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Long Term Stability of Light Sources

PhotoFluor II (blue) and 120 W metal halide source (gray) measured at microscope stage over 14 hours. Power meter measured output of a field of fluorescent beads (Tetraspecks) using a 49004 filter set (excitation from 533 nm to 558 nm), sampling every 5 seconds. Initial brightness of PhotoFluor II was decreased to match that of the 120 W light source. 20 X Zeiss objective used with a 0.5 ND filter.