TwinCam LS Image Splitter

Affordable and user-friendly image splitter that enables two-camera, simultaneous dual-emission imaging from a single microscope port

  • Easy to align and maintain
  • Ideal for FRET and wide-field large sensor simultaneous dual-channel imaging

The TwinCam LS Image Splitter has been designed as a convenient solution for simultaneous two-channel imaging without sacrificing field of view. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially.  However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the TwinCam LS allows two images to be acquired simultaneously.

This image splitter enables pixel alignment between two cameras coupled to a single microscope port. The base unit accommodates a standard Cairn filter cube allowing light to be distributed on the basis of wavelength, polarization state, or focal depth. Each cube has fine mechanical X-Y adjustment for pixel overlay or deliberate image offset. The input of the instrument has a variable rectangular aperture enabling the use of cropped sensor mode on both detectors.

Standard spectral range

450 nm – 900 nm


348 mm (L) x 191.5 mm (W) x 72 mm (H)


1.8 kg

Emission filter size

25 mm

Dichroic mirror size

25.5 mm x 36.0 mm
26.0 mm x 38.0 mm

2.0 mm thick, UltraFlat dichroics highly recommended

Camera size supported

Up to 22 mm diagonal

Supports large sensors up to 22 mm diagonal

Enables the largest FOV possible

User exchangeable dichroic mirrors

Easily reconfigure system for different experiments

Adjustable input aperture

Precisely define the required field of view; also supports cropped sensor mode found in many EMCCD cameras

Supports dichroic mirrors up to 3 mm thick

Ensures a flat reflection surface and distortion-free images

Stable alignment

Enables long-term time-lapse imaging without the need to realign during the experiment

Optional auxiliary filter holders

Ability to balance emission intensity between the multiple channels or add emission; accommodates ND filters and chromatic correction lenses

Independent camera focus

Corrects chromatic aberrations from the microscope

  • Förster Resonance Energy Transfer (FRET)
  • Simultaneous use of multiple dyes or genetic markers
  • Ratiometric imaging
  • Polarization studies
  • Simultaneous transmitted light and fluorescence imaging
  • Simultaneous high speed and high resolution imaging
  • Simultaneous imaging of two Z depths

Additional Information

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