OptoSplit II LS Image Splitter

Easy-to-align image splitter that enables a single camera to record images simultaneously at two different emission wavelengths

  • User-friendly design requires minimal maintenance and makes alignment simple
  • Excellent for use in FRET, TIRF, and spinning disk confocal applications
  • Compatible with all major brands of camera, including large sCMOS chips

The OptoSplit II LS has been designed as a convenient, inexpensive solution for simultaneous dual wavelength imaging. Traditionally, dual-wavelength imaging experiments required that two images be captured sequentially.  However, because the two images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures.  To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit II allows both images to be acquired simultaneously.

The images captured by the OptoSplit II contain information from two different emission bands, and can be processed using commercially available software packages. Alternatively, the images can be processed manually.  While it is optimized for coupling to a scientific microscope, the OptoSplit II can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size.

Standard Spectral Range

450 nm – 900 nm


110 mm (L) x 261 mm (W) x 79 mm (H)


1.26 kg

Emission filter size

25 mm

Dichroic mirror size

25.5 mm x 36.0 mm
26.0 mm x 38.0 mm

2.0 mm thick UltraFlat dichroics highly recommended

Camera size supported

Up to 22 mm diagonal

Supports large sensors up to 22 mm diagonal

Enables large field of view

User exchangeable dichroic mirrors

Easily reconfigure system for different experiments

Adjustable input aperture

Ability to precisely define the required field of view; also supports cropped sensor mode found in many EMCCD cameras

Supports dichroic mirrors up to 3 mm thick

Ensures distortion-free images

Stable alignment

Enables long-term time-lapse imaging without the need to realign during an experiment

Optional auxiliary filter holders

Ability to balance emission intensity between the multiple channels or add emission; accommodates ND filters and chromatic correction lenses

  • Ratiometric, calcium, voltage & pH imaging
  • Dual probe widefield imaging
  • Förster Resonance Energy Transfer (FRET)
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal microscopy
  • Polarization/Anisotropy
  • Combined fluorescence/transmitted light microscopy


Additional Information

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