OptoSpin IV – High Speed Filter Wheel

Compact filter wheel provides high-speed stepping without vibration

  • Offers continuous spinning over a wide range of speeds and between filter positions
  • Two filter wheels can be combined to economically replace a traditional 10-position filter wheel
  • Excellently-suited for high-speed applications

Tradition epiflourescence requires optical filter cubes to be positioned in a turret inside the microscope. However, filter-switching time is too long to allow for observation of many biological processes. The addition of an Optospin IV filter wheel allows excitation and emission filters to be changed rapidly (approximately 25 milliseconds).  This rapid changing of filters improves the time resolution of FRET and ratiometric imaging techniques.

The Cairn Optospin IV has been designed for compact and flexible operation. The OptoSpin IV’s motor is mounted directly within the hub of the filter wheel, which both avoids the “motor bulge” present on other systems and minimizes the inertial losses associated with geared connections.  Equipped with a powerful new motor, the OptoSpin IV achieves low response times while still supporting 25 mm filters. The OptoSpin IV also delivers continuous spinning over a wide range of speeds and is able to step between filter positions. Continuous spinning allows wavelengths to be changed on a millisecond timescale, as required for high-speed imaging, and further reduces the vibration problems associated with stepwise operation.

For maximal functionality, the OptoSpin IV has been standardized on a six-filter wheel, but it is offset within its housing so that two housings can be joined in an interlocking fashion. This design allows both wheels to be incorporated within the same overall optical and mechanical path length of 35mm. This in turn allows the controller box to treat this combination as a single ten-position filter wheel (five active positions and one open position in each one), but with substantially reduced inertia – and hence stepping time – as compared to a single ten-position filter wheel.

The controller box can drive up to four filter wheels simultaneously and can interconnect with other equipment by either a digital or a USB interface. In spin mode, any or all wheels can be spun in both speed and phase synchronization. In step mode, wheels can be simultaneously but independently stepped between any combination of filter positions.

Filter positions

6 (or 10 with using 2 interlocking filter wheels)

Filter size

25 mm

Stepping time

<30 msec adjacent
<50 msec opposite

Spin rate

10,000 RPM (1 msec/filter)

Dimensions

100 mm x 100 mm x 25 mm

Control options

USB or TTL

FeatureBenefit
Stepping times as low as 30msec between adjacent filters

Support for high-speed imaging applications

Continuous spinning up to 10,000 rpm

Enables high-speed excitation ratiometric imaging

USB and TTL control

Flexibility; supports any software capable of outputting TTLs

  • Multi channel fluorescence imaging
  • High speed ratiometric imaging
  • FRET
  • Spectrophotometry
  • Low-vibration microscopy

Additional Information


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